Protective effect of trans-nerolidol on vascular endothelial cell injury induced by lipopolysaccharides

Abstract

This article aimed to discuss the protection of trans-nerolidol on vascular endothelial cells (ECs) injured by lipopolysaccharides. ECs were divided into four groups: normal, model, low and high dose trans-nerolidol treatment groups. The cell survival rate and the contents of NO in the cell culture
supernatant were determined. The protein expression and transcript level of peroxisome proliferatoractivated receptor-γ (PPARγ), endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) were determined by western blotting and RT-PCR respectively. Compared with the
normal group, cell livability, protein expression and mRNA transcript level of PPARγ and eNOS decreased, NO contents, protein expression and mRNA transcript tlevel of iNOS increased in model group significantly. Compared with model group, all the changes recovered in different degree in
treatment groups. Hence, it was concluded that trans-nerolidol can alleviate the ECs injuryby the regulation of iNOS/eNOS through activating PPARγ in a dose-dependent manner.