In vitro propagation of Anredera vesicaria

Abstract

Anredera vesicaria is a plant whit high anti-inflammatory activity. The work objective was to establish in vitro propagation of A. vesicaria. Nodal segments were used as explants and two disinfection times in 1% sodium hypochlorite (15 and 20 minutes) were evaluated during in vitro establishment.
Combinations of AG3 (2,5 and 5,0 mg/L) and IAA (0,05 and 0,1 mg/L) were evaluated in the multiplication phase and the effect of IBA (0,5 and 1,0 mg /L) and Pectimorf (1,0 and 5,0 mg/L) for in vitro rooting. Acclimatization was carried out in a mixture of soil-cow manure-zeolite. Disinfection was achieved with 1% sodium hypochlorite for 15 minutes and in vitro establishment in the MS (1962) culture medium. High values of multiplication and rooting in vitro were obtained, as well as acclimatization of plants in vitro.