Non-volatile leaves compounds LC-MS profile of the two Cuban Piper aduncum L. subspecies

Abstract

The medicinal plant Piper aduncum was segregated in Cuba as P. aduncum subsp. ossanum and P. aduncum subsp. aduncum, the latter has not been phytochemically investigated in Cuba. This study aims to compare profiles of non-volatile compounds of the aqueous and ethanolic extracts of Cuban Piper
aduncum subspecies leaves. From both subspecies were prepared a decoction and, an ethanolic extract that yield two fractions. Analysis was performed by UPLC-ESI/QTOF/MS. Standard reference compounds were assessed by comparison with MS data of the plants extracts. Also, tentatively assigned
compounds were proposed by matching m/z and fragmentation patterns with P. aduncum published compounds. Main compounds in decoction and aqueous ethanolic fraction of both subspecies were characterized as flavon-C-glycosides. Isoorientin was not previously reported in P. aduncum. Oleaginous
ethanolic extracts differs among subspecies and peaks are coincident with benzoic acid derivate compounds. Results can serve for quality control of P. aduncum in Cuba.