Study of the chloroformic extract of Gnaphalium sp in a model of alloxaninduced diabetes in Wistar rats. An alternative use of the “gordolobo” plant within traditional Mexican medicine

Abstract

“Gordolobo” (Gnaphalium sp) is a Mexican medicinal plant understudied for the treatment of diabetes; therefore, the aim was to evaluate the chloroformic extract of G sp. (CEG) in alloxan-induced diabetic rats. Sesquiterpene lactones, polyphenolic compounds, triterpenes and steroids, apigenin, and lauric and myristic acid were identified in CEG by phytochemical, HPLC and GC-MS analysis; and the antioxidant capacity evaluated by FRAP, DPPH and ABTS, inhibited the formation of free radicals. There was no lethality or toxicity at doses of 2000 mg/kg. At doses of 200 mg/kg it did not decrease hyperglycemia; however, it did decrease biomarkers of oxidative stress (malondialdehyde, oxidized proteins, superoxide dismutase) associated with diabetes in pancreas. The β-cell function, insulin resistance and insulin sensitivity were not improved. In conclusion, CEG showed no hypoglycemic activity, but antioxidant activity in pancreatic tissue.