in-vitro Cytotoxicity/Anti-Radical Capability and structural characterization by GC-MS/1H-13C- NMR of the leaves essential oils of young/old trees of Annona purpurea Moc. & Sessé ex Dunal from Repelón (Atlántico, Colombia)

Abstract

Annona purpurea is a plant used in ethnomedicine in the northern region of Colombia, which has no scientific reports on the composition of their essential oil (EO) and biological activities. In this work, the leaves EO of young/old trees were characterized by GC-MS and NMR, and their antiradical/cytotoxic properties were evaluated. β-Eudesmol (68.9%) and α-eudesmol (16.8%), and germacrene D (55.6%) and bicyclogermacrene (20.3%), were the representative compounds of the leaves EO of young/old trees, respectively. The NMR analysis showed the distinctive signals of the main constituents identified. The TAA values (mmol Trolox®/kg ES) obtained from the EO of young/old trees were 165 ± 8 and 602 ± 38. The EO of young/old trees evaluated on human lymphocytes were “moderately toxic” with LC50 (μg/mL) of 145.5 ± 0.7 and 346 ± 8. Finally, the cytotoxicity in human erythrocytes revealed that the old tree EO was not haemolytic (LC50 > 1000 μg/mL, 4.3 ± 0.6%); while the young tree EO was hemolytic (LC50 490 ± 48 μg/mL).